BTEC 300. Introduction to Biotechnology. 3 Hours N.
Review of techniques used in food, agricultural, pharmaceutical, industrial, and environmental biotechnology. Role of regulatory agencies during the discovery, development, and manufacture of new medical devices, biotechnology, biomedical, and pharmaceutical products. Guest presentations in biotechnology. Prerequisite: BIOL 416. LEC.
BTEC 330. Biotechnology Regulation and Documentation Processes. 3 Hours N.
Current good manufacturing practices (GMP) as they apply in the biotechnology workplace. History, rationale, purpose, and GMP requirements applicable to the manufacturing, packaging, labeling, testing, and control of pharmaceutical products, and consequences of inaction. Prerequisite: BTEC 300. LEC.
BTEC 340. Biotechnology Research Methods and Applications. 3 Hours N.
Introduction to fermentation and protein chemistry. Theory behind laboratory techniques and overview of industrial scale expression systems. Bacterial cell culture techniques, principles of fermenter operation and purification, documentation procedures, important tasks for clean room operations, including sanitization, sterilization, cleaning procedures, calibration, and environmental monitoring. Prerequisite: BTEC 300; BIOL 600. LEC.
BTEC 341. Principles of Bioprocessing Laboratory I. 1 Hour N.
Laboratory sessions involve use of microbial expression vectors, fermentation systems, and large-scale purification of recombinant protein. Includes bacterial cell culture techniques, principles of bioreactor/fermentation operations and purification techniques, and calibration. Primary goal of this course is to provide students with an advanced background in bacterial upstream and downstream biotechnology. Prerequisite: BTEC 300; BTEC 340 or concurrent enrollment in BTEC 340. LAB.
BTEC 405. Microbial Genetics Laboratory. 4 Hours N.
Research projects center on using molecular genetics to examine the biology of the bacterium Pseudomonas aeruginosa, an opportunistic pathogen often found in the lungs of cystic fibrosis patients. Students engage in independent projects to probe various aspects of P. aeruginosa physiology such as antibiotic resistance, phase variation, toxin production, secondary metabolite production, twitching motility, swarming behaviors, and more. Projects aim to discover the molecular basis for these processes using both classical and new, cutting-edge techniques. These include plasmid manipulation, genetic complementation, mutagenesis, PCR, DNA sequencing, enzyme assays, and gene expression studies. Prerequisite: BIOL 350; BIOL 402 . LAB.
BTEC 424. Independent Study in Biotechnology. 1-3 Hours N.
Independent project at a related bioscience industry partner or faculty in selected topics of current translational research interest. May be undertaken only with the consent of the major advisor who will guide the research after determining objectives with the interested industry partner or faculty. Prerequisite: Consent of instructor. IND.
BTEC 440. Biotechnology Research Methods and Applications II. 3 Hours N.
Theory and practicum behind laboratory techniques and overview of industrial scale expression systems of insect or mammalian protein chemistry. Cell culture techniques, principles of bioreactor operation and purification, documentation procedures, important tasks for clean room operations, including sanitization, sterilization, cleaning procedures, calibration, and environmental monitoring are evaluated. Prerequisite: BTEC 340; BTEC 341. LEC.
BTEC 441. Principles of Bioprocessing Laboratory II. 1 Hour N.
Mammalian cell culture techniques, principles of bioreactor operations and purification techniques, and calibration. The primary goal of this course is to provide students with an advanced background in mammalian upstream and downstream biotechnology. Prerequisite: BTEC 341; BTEC 440 or concurrent enrollment in BTEC 440. LAB.
BTEC 450. Applied Bioinformatics. 3 Hours N.
Overview of the fields of bioinformatics and genomics. Topics, tools, issues and current trends in these and related fields are discussed. Principles and practical application of bioinformatics tools in molecular biology and genetics are evaluated. The haploid human genome occupies a total of just over 3 billion DNA base pairs. This information is not contained in books, but stored in electronic databases. Computational biology utilizes infer function by comparative analysis. This course is designed for life scientists from all fields to introduce them to the power of bioinformatics and enable them to access and utilize biological information in databases for their own research. Prerequisite: BTEC 300; BIOL 570 or MATH 365 or PSYC 210. LEC.
BTEC 460. Introduction to Quality Control/Quality Assurance in Biotechnology. 3 Hours N.
Quality control techniques, assurance issues, and management methods. Quality in design and planning, in the constructed project, and in production of goods and services. Prerequisite: BTEC 330. LEC.
BTEC 475. Bioseparations Laboratory. 2 Hours N.
Develop novel and effective strategies for extraction and purification of recombinant and native biomolecules by understanding constraints posed by the biological system and the products. Research projects are geared toward developing cost-effective processes for recovery of industrial and biopharmaceutical products derived from a variety of native and/or transgenic sources. Prerequisite: BTEC 405; BTEC 441. LAB.
BTEC 494. Selected Topics in Biotechnology. 1 Hour N.
Course work varies with the topic of the seminar. The preparation and presentation of oral reports on selected topics from recent translational research literature. Students may choose one interest group each semester, but may enroll in a given interest group only once. May be repeated for credit when topics vary. Prerequisite: BTEC 300 and approval of instructor. LEC.
BTEC 501. Ethical Issues in Biotechnology. 1 Hour N.
Student investigations and discussions of current controversial issues in biotechnology. This course emphasizes thinking about new technologies in a rational and thoughtful way. Prerequisite: BTEC 300. LEC.
BTEC 541. Gene Expression Analysis: Microarrays. 2 Hours N.
This course reviews current theory, techniques, instrumentation, troubleshooting, analysis tools, and advanced protocols for microarray analysis. Students have the opportunity to utilize skills learned during lecture in a laboratory environment. At the conclusion of this course, students understand microarray experimental design, its tools, and analysis of generated data. Prerequisite: BTEC 300. LAB.
BTEC 542. Protein Expression in Insect Cells. 2 Hours N.
Introduction to the insect cells expression system, and its advantages and disadvantages. Introduction to expression of recombinant proteins with baculovirus. Outline of antibody and antibody fragments as well as other complex proteins. Basic techniques used for growth and maintenance of insect cell cultures. The lab portion of the course provides students with practical experience in protein expression techniques in the insect cells expression system. Prerequisite: BTEC 300. LAB.
BTEC 545. RNA Interference and Model Organisms. 2 Hours N.
Introduction and history of RNA interference technology. Principles, mechanism, and applications of RNA interference in model organisms. Laboratory sessions include RNA interference-mediated silencing of genes in plants, C. elegans, and mammalian cell culture. Prerequisite: BTEC 300. LAB.
BTEC 547. Bioanalytical Lab. 2 Hours N.
Analytical methods used for testing biotherapeutics are examined. Emphasis is placed on assessing protein concentration, purity, identity and activity. The importance of sample processing, throughput and level of validation are explored as samples from upstream processing, downstream processing and final bulk are interrogated. Students also learn key concepts used to validate the performance of analytical methods. Prerequisite: BTEC 300. LAB.
BTEC 599. Biotechnology Capstone Experience. 3 Hours N.
Supervised internship at a biotech company; or an independent thesis; or honors thesis with Honors Program. Prerequisite: BTEC 441 and approval of instructor. FLD.